Chromatin accessibility dynamics across C. elegans development and ageing
† Equal contribution
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Abstract
An essential step for understanding the transcriptional circuits that control development and physiology is the global identification and characterization of regulatory elements. Here, we present the first map of regulatory elements across the development and ageing of an animal, identifying 42,245 elements accessible in at least one Caenorhabditis elegans stage. Based on nuclear transcription profiles, we define 15,714 protein-coding promoters and 19,231 putative enhancers, and find that both types of element can drive orientation-independent transcription. Additionally, more than 1000 promoters produce transcripts antisense to protein coding genes, suggesting involvement in a widespread regulatory mechanism. We find that the accessibility of most elements changes during development and/or ageing and that patterns of accessibility change are linked to specific developmental or physiological processes. The map and characterization of regulatory elements across C. elegans life provides a platform for understanding how transcription controls development and ageing.
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Jänes J, Dong Y, Schoof M, Serizay J, Appert A, Cerrato C, et al. Chromatin accessibility dynamics across C. elegans development and ageing. eLife. 2018;7:e37344. doi:10.7554/eLife.37344
@article{janes_elife_2018,
author = {Jänes, Jürgen and Dong, Yan and Schoof, Michael and Serizay, Jacques and Appert, Alex and Cerrato, Chiara and Woodbury, Carson and Chen, Ron and Gemma, Carolina and Huang, Ni and Kissiov, Djem and Stempor, Przemyslaw and Steward, Annette and Zeiser, Eva and Sauer, Sascha and Ahringer, Julie},
title = {{Chromatin accessibility dynamics across C. elegans development and ageing}},
journal = {eLife},
year = {2018},
volume = {7},
pages = {e37344},
doi = {10.7554/eLife.37344},
url = {https://doi.org/10.7554/eLife.37344}
}