Epigenomics Workshop

Welcome

Package: NGSPhysalia
Authors: Jacques Serizay [aut, cre]
Compiled: 2026-04-16
Package version: 0.99.0
R version: R Under development (unstable) (2026-04-12 r89873)
BioC version: 3.23
License: MIT + file LICENSE

This is the landing page for the β€œEpigenomics Data Analysis” workshop, ed. 2026.

What

This course will introduce biologists and bioinformaticians to the field of regulatory epigenomics. We will cover a range of software and analysis workflows for processing and quantitative analysis of ChIP-seq, MNase and ATAC-seq data, RNA-seq, and Hi-C data. We will start by introducing general concepts about epigenomics. From there, we will then continue to describe the main analysis steps to go from raw sequencing data to processed and usable data. We will present classical analysis workflows, their output and the possible paths to investigate downstream of this. Towards the end of the workshop, we will focus on multi-omics data integration. Throughout the workshop, real-world datasets will be analysed using bash tools and R/Bioconductor packages.

Target audience

This course is primarily targeting researchers who are relatively new to the field of bioinformatics, with practical experience in the experimental side of epigenomics. Attendees should have a background in biology as well as be familiar with genomic data and common file formats from NGS sequencing experiments (fastq, BAM, BED).

Practical exercises will use command-line Linux and R code and will be presented as notebooks to ensure reproducible coding. Attendees are expected to know the basics of the R programming language and have prior expe- rience with the Unix command line.

Why

By the end of the course, participants will be able to:

  • Understand the principles and experimental designs of key genomic assays.
  • Perform preprocessing, alignment, and quality control for RNA-seq, ATAC-seq, ChIP-seq, and Hi-C data.
  • Conduct downstream analyses such as differential expression, peak calling, motif analysis, and interaction visualization.
  • Integrate multiple genomic data types to derive biological insights.

Instructors

Jacques Serizay

Session info

sessioninfo::session_info(
    installed.packages()[,"Package"], 
    include_base = TRUE
)
##  ─ Session info ────────────────────────────────────────────────────────────
##   setting  value
##   version  R Under development (unstable) (2026-04-12 r89873)
##   os       Ubuntu 24.04.4 LTS
##   system   x86_64, linux-gnu
##   ui       X11
##   language (EN)
##   collate  en_US.UTF-8
##   ctype    en_US.UTF-8
##   tz       Etc/UTC
##   date     2026-04-16
##   pandoc   3.9.0.2 @ /usr/bin/ (via rmarkdown)
##   quarto   1.9.37 @ /usr/local/bin/quarto
##  
##  ─ Packages ────────────────────────────────────────────────────────────────
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##   RcppEigen                           0.3.4.0.2 2024-08-24 [1] CRAN (R 4.7.0)
##   RcppHNSW                            0.6.0     2024-02-04 [1] CRAN (R 4.7.0)
##   RcppProgress                        0.4.2     2020-02-06 [1] CRAN (R 4.7.0)
##   RcppThread                          2.3.0     2026-01-16 [1] CRAN (R 4.7.0)
##   RcppTOML                            0.2.3     2025-03-08 [1] CRAN (R 4.7.0)
##   RCurl                               1.98-1.18 2026-03-21 [1] CRAN (R 4.7.0)
##   readr                               2.2.0     2026-02-19 [1] CRAN (R 4.7.0)
##   readxl                              1.4.5     2025-03-07 [1] CRAN (R 4.7.0)
##   rebook                              1.21.0    2025-11-02 [1] Bioconduc~
##   rematch                             2.0.0     2023-08-30 [1] CRAN (R 4.7.0)
##   rematch2                            2.1.2     2020-05-01 [1] CRAN (R 4.7.0)
##   renv                                1.2.2     2026-04-16 [1] CRAN (R 4.7.0)
##   reprex                              2.1.1     2024-07-06 [1] CRAN (R 4.7.0)
##   reshape2                            1.4.5     2025-11-12 [1] CRAN (R 4.7.0)
##   restfulr                            0.0.16    2025-06-27 [1] CRAN (R 4.7.0)
##   reticulate                          1.46.0    2026-04-09 [1] CRAN (R 4.7.0)
##   rhdf5                               2.55.16   2026-03-12 [1] Bioconduc~
##   rhdf5filters                        1.23.3    2025-12-07 [1] Bioconduc~
##   Rhdf5lib                            1.33.6    2026-03-16 [1] Bioconduc~
##   Rhtslib                             3.7.0     2025-10-30 [1] Bioconduc~
##   rjson                               0.2.23    2024-09-16 [1] CRAN (R 4.7.0)
##   rlang                               1.2.0     2026-04-06 [1] CRAN (R 4.7.0)
##   rmarkdown                           2.31      2026-03-26 [1] CRAN (R 4.7.0)
##   ROCR                                1.0-12    2026-01-23 [1] CRAN (R 4.7.0)
##   roxygen2                            7.3.3     2025-09-03 [1] CRAN (R 4.7.0)
##   rpart                               4.1.27    2026-03-27 [2] CRAN (R 4.7.0)
##   rprojroot                           2.1.1     2025-08-26 [1] CRAN (R 4.7.0)
##   Rsamtools                           2.27.2    2026-04-09 [1] Bioconduc~
##   RSpectra                            0.16-2    2024-07-18 [1] CRAN (R 4.7.0)
##   RSQLite                             2.4.6     2026-02-06 [1] CRAN (R 4.7.0)
##   rstudioapi                          0.18.0    2026-01-16 [1] CRAN (R 4.7.0)
##   rtracklayer                         1.71.3    2025-12-14 [1] Bioconduc~
##   Rtsne                               0.17      2023-12-07 [1] CRAN (R 4.7.0)
##   rversions                           3.0.0     2025-10-09 [1] CRAN (R 4.7.0)
##   rvest                               1.0.5     2025-08-29 [1] CRAN (R 4.7.0)
##   S4Arrays                            1.11.1    2025-11-25 [1] Bioconduc~
##   S4Vectors                           0.49.1-1  2026-04-05 [1] Bioconduc~
##   S7                                  0.2.1-1   2025-11-14 [1] CRAN (R 4.7.0)
##   sass                                0.4.10    2025-04-11 [1] CRAN (R 4.7.0)
##   scales                              1.4.0     2025-04-24 [1] CRAN (R 4.7.0)
##   scattermore                         1.2       2023-06-12 [1] CRAN (R 4.7.0)
##   scatterpie                          0.2.6     2025-09-12 [1] CRAN (R 4.7.0)
##   sctransform                         0.4.3     2026-01-10 [1] CRAN (R 4.7.0)
##   selectr                             0.5-1     2025-12-17 [1] CRAN (R 4.7.0)
##   Seqinfo                             1.1.0     2025-10-31 [1] Bioconduc~
##   seqLogo                             1.77.0    2025-10-30 [1] Bioconduc~
##   sessioninfo                         1.2.3     2025-02-05 [1] CRAN (R 4.7.0)
##   Seurat                              5.4.0     2025-12-14 [1] CRAN (R 4.7.0)
##   SeuratObject                        5.4.0     2026-04-11 [1] CRAN (R 4.7.0)
##   shiny                               1.13.0    2026-02-20 [1] CRAN (R 4.7.0)
##   SingleCellExperiment                1.33.2    2026-03-24 [1] Bioconduc~
##   sitmo                               2.0.2     2021-10-13 [1] CRAN (R 4.7.0)
##   snow                                0.4-4     2021-10-27 [1] CRAN (R 4.7.0)
##   sourcetools                         0.1.7-2   2026-03-28 [1] CRAN (R 4.7.0)
##   sp                                  2.2-1     2026-02-13 [1] CRAN (R 4.7.0)
##   spam                                2.11-3    2026-01-08 [1] CRAN (R 4.7.0)
##   SparseArray                         1.11.13   2026-04-01 [1] Bioconduc~
##   spatial                             7.3-18    2025-01-01 [2] CRAN (R 4.7.0)
##   SpatialExperiment                   1.21.0    2025-11-03 [1] Bioconduc~
##   spatstat.data                       3.1-9     2025-10-18 [1] CRAN (R 4.7.0)
##   spatstat.explore                    3.8-0     2026-03-22 [1] CRAN (R 4.7.0)
##   spatstat.geom                       3.7-3     2026-03-23 [1] CRAN (R 4.7.0)
##   spatstat.random                     3.4-5     2026-03-22 [1] CRAN (R 4.7.0)
##   spatstat.sparse                     3.1-0     2024-06-21 [1] CRAN (R 4.7.0)
##   spatstat.univar                     3.1-7     2026-03-18 [1] CRAN (R 4.7.0)
##   spatstat.utils                      3.2-2     2026-03-10 [1] CRAN (R 4.7.0)
##   splines                             4.7.0     2026-04-13 [2] local
##   stats                             * 4.7.0     2026-04-13 [2] local
##   stats4                              4.7.0     2026-04-13 [2] local
##   strawr                              0.0.92    2024-07-16 [1] CRAN (R 4.7.0)
##   stringi                             1.8.7     2025-03-27 [1] CRAN (R 4.7.0)
##   stringr                             1.6.0     2025-11-04 [1] CRAN (R 4.7.0)
##   SummarizedExperiment                1.41.1    2026-02-06 [1] Bioconduc~
##   survival                            3.8-6     2026-01-16 [2] CRAN (R 4.7.0)
##   sys                                 3.4.3     2024-10-04 [1] CRAN (R 4.7.0)
##   systemfonts                         1.3.2     2026-03-05 [1] CRAN (R 4.7.0)
##   tcltk                               4.7.0     2026-04-13 [2] local
##   tensor                              1.5.1     2025-06-17 [1] CRAN (R 4.7.0)
##   testthat                            3.3.2     2026-01-11 [1] CRAN (R 4.7.0)
##   textshaping                         1.0.5     2026-03-06 [1] CRAN (R 4.7.0)
##   TFBSTools                           1.49.0    2025-11-04 [1] Bioconduc~
##   TFMPvalue                           1.0.0     2026-01-19 [1] CRAN (R 4.7.0)
##   tibble                              3.3.1     2026-01-11 [1] CRAN (R 4.7.0)
##   tidyCoverage                        1.7.1     2026-01-07 [1] Bioconduc~
##   tidydr                              0.0.6     2025-07-25 [1] CRAN (R 4.7.0)
##   tidygate                            1.0.19    2025-12-10 [1] CRAN (R 4.7.0)
##   tidyomics                           1.7.0     2025-11-04 [1] Bioconduc~
##   tidyr                               1.3.2     2025-12-19 [1] CRAN (R 4.7.0)
##   tidyselect                          1.2.1     2024-03-11 [1] CRAN (R 4.7.0)
##   tidyseurat                          0.8.10    2026-02-26 [1] CRAN (R 4.7.0)
##   tidySingleCellExperiment            1.21.0    2025-11-03 [1] Bioconduc~
##   tidySpatialExperiment               1.7.2     2025-12-01 [1] Bioconduc~
##   tidySummarizedExperiment            1.21.0    2025-10-31 [1] Bioconduc~
##   tidytree                            0.4.7     2026-01-08 [1] CRAN (R 4.7.0)
##   tidyverse                           2.0.0     2023-02-22 [1] CRAN (R 4.7.0)
##   timechange                          0.4.0     2026-01-29 [1] CRAN (R 4.7.0)
##   tinytex                             0.59      2026-03-28 [1] CRAN (R 4.7.0)
##   tools                               4.7.0     2026-04-13 [2] local
##   treeio                              1.35.0    2025-10-30 [1] Bioconduc~
##   ttservice                           0.5.3     2025-07-10 [1] CRAN (R 4.7.0)
##   tweenr                              2.0.3     2024-02-26 [1] CRAN (R 4.7.0)
##   TxDb.Celegans.UCSC.ce11.ensGene     3.15.0    2026-04-16 [1] Bioconductor
##   TxDb.Hsapiens.UCSC.hg19.knownGene   3.22.1    2026-04-16 [1] Bioconductor
##   tzdb                                0.5.0     2025-03-15 [1] CRAN (R 4.7.0)
##   UCSC.utils                          1.7.1     2025-12-09 [1] Bioconduc~
##   universalmotif                      1.29.2    2026-04-10 [1] Bioconduc~
##   urlchecker                          1.0.1     2021-11-30 [1] CRAN (R 4.7.0)
##   usethis                             3.2.1     2025-09-06 [1] CRAN (R 4.7.0)
##   utf8                                1.2.6     2025-06-08 [1] CRAN (R 4.7.0)
##   utils                             * 4.7.0     2026-04-13 [2] local
##   uuid                                1.2-2     2026-01-23 [1] CRAN (R 4.7.0)
##   uwot                                0.2.4     2025-11-10 [1] CRAN (R 4.7.0)
##   vctrs                               0.7.3     2026-04-11 [1] CRAN (R 4.7.0)
##   vipor                               0.4.7     2023-12-18 [1] CRAN (R 4.7.0)
##   viridis                             0.6.5     2024-01-29 [1] CRAN (R 4.7.0)
##   viridisLite                         0.4.3     2026-02-04 [1] CRAN (R 4.7.0)
##   vroom                               1.7.1     2026-03-31 [1] CRAN (R 4.7.0)
##   waldo                               0.6.2     2025-07-11 [1] CRAN (R 4.7.0)
##   whisker                             0.4.1     2022-12-05 [1] CRAN (R 4.7.0)
##   withr                               3.0.2     2024-10-28 [1] CRAN (R 4.7.0)
##   xfun                                0.57      2026-03-20 [1] CRAN (R 4.7.0)
##   XML                                 3.99-0.23 2026-03-20 [1] CRAN (R 4.7.0)
##   xml2                                1.5.2     2026-01-17 [1] CRAN (R 4.7.0)
##   xopen                               1.0.1     2024-04-25 [1] CRAN (R 4.7.0)
##   xtable                              1.8-8     2026-02-22 [1] CRAN (R 4.7.0)
##   XVector                             0.51.0    2025-10-31 [1] Bioconduc~
##   yaml                                2.3.12    2025-12-10 [1] CRAN (R 4.7.0)
##   yulab.utils                         0.2.4     2026-02-02 [1] CRAN (R 4.7.0)
##   zip                                 2.3.3     2025-05-13 [1] CRAN (R 4.7.0)
##   zoo                                 1.8-15    2025-12-15 [1] CRAN (R 4.7.0)
##  
##   [1] /usr/local/lib/R/site-library
##   [2] /usr/local/lib/R/library
##   * ── Packages attached to the search path.
##  
##  ─ Python configuration ────────────────────────────────────────────────────
##   Python is not available
##  
##  ───────────────────────────────────────────────────────────────────────────
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